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Anti-CAMKII alpha Antibody (4A308) is a Rabbit antibody targeting CAMKII alpha. Anti-CAMKII alpha Antibody (4A308) can be used in FCM,IP,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-CAMKII alpha Antibody (4A308) is a Rabbit antibody targeting CAMKII alpha. Anti-CAMKII alpha Antibody (4A308) can be used in FCM,IP,WB. |
Synonyms | zgc:123320, zgc:112538, R74975, PK2CDD, mKIAA0968, MGC155201, MGC139375, MGC123320, KIAA0968, KCC2A, EC 2.7.11.17, CaMK-II subunit alpha, CaMKII alpha, CaMKII, CAMKB, CAMKA, CAMK2D, CAMK2A, CaMK II delta subunit, CaMK II beta subunit, CaMK II alpha subunit, CAM2, CaM kinase II subunit alpha, CaM kinase II delta subunit, CaM kinase II delta chain, CaM kinase II beta subunit, CaM kinase II beta chain, CaM kinase II alpha subunit, CaM kinase II alpha chain, Calcium/calmodulin-dependent protein kinase(CaM kinase) II alpha, Calcium/calmodulin-dependent protein kinase type IIA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/Calmodulin Dependent Protein Kinase II G, Calcium/calmodulin dependent protein kinase II alpha, Calcium calmodulin dependent protein kinase II alpha-B subunit, Calcium calmodulin dependent protein kinase II, Calcium calmodulin dependent protein kinase CaM kinase II alpha, alpha CaMKII |
Ig Type | IgG |
Clone | 4A308 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution. 2. Flow cytometric analysis of SH-SY5Y cells with CaMKII alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the _x0018_secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000-2000; IP: 1:10-50; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 54 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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