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Anti-CAMK2G Antibody (8A304) is a Mouse antibody targeting CAMK2G. Anti-CAMK2G Antibody (8A304) can be used in WB,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $426 | 7-10 days |
Description | Anti-CAMK2G Antibody (8A304) is a Mouse antibody targeting CAMK2G. Anti-CAMK2G Antibody (8A304) can be used in WB,IHC,FCM. |
Synonyms | KCC2A_HUMAN, CaMK-II subunit alpha, CAMKA, CAMK2G, CAMK2D, CAMK2B, CAMK2A, CAMK2, CaM kinase II subunit alpha, CaM kinase II gamma, CaM kinase II delta, CaM kinase II beta, CaM kinase II alpha, CaM kinase II, Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/calmodulin dependent protein kinase II gamma, Calcium/calmodulin dependent protein kinase II delta, Calcium/calmodulin dependent protein kinase II beta, Calcium/calmodulin dependent protein kinase II alpha |
Reactivity | Human,Rat |
Verified Activity | 1. Western blot analysis of CaMKII gamma on human CaMKII gamma recombinant protein using anti- CaMKII gamma antibody at 1/1,000 dilution. 2. Western blot analysis of CaMKII gamma on HEK293 (1) and CaMKII gamma-hIgGFc transfected HEK293 (2) cell lysate using anti- CaMKII gamma antibody at 1/1,000 dilution. 3. Western blot analysis of CaMKII gamma on different cell lysate using anti-CaMKII gamma antibody at 1/1,000 dilution. Positive control: Line1: PC-12, Line2: Jurkat, Line3: T47D, Line4: HepG2. 4. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CaMKII gamma antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CaMKII gamma antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of Jurkat cells with CaMKII gamma antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:200-500; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 63 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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