Anti-CAMK2 beta Antibody (7C496) is a Mouse antibody targeting CAMK2 beta. Anti-CAMK2 beta Antibody (7C496) can be used in WB,IHC-P,IHC-Fr,IF.

IgG1

7C496

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (human cerebellum tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CAMK2 beta) Monoclonal Antibody, Unconjugated (TMAB-03590) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: Jurkat cell lysates
Lane 2: PC-12 cell lysates
Lane 3: Human brain tissue lysates
Lane 4: Mouse Heart tissue lysates
Primary: Anti-CAMK2 beta (TMAB-03590) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

Recombinant Protein: human CAMK2 between 1-503 amino acids

Human

CAMK2B

Calcium/calmodulin-dependent protein kinase type II subunit beta

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplamic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.