Anti-Calreticulin Antibody (8J512) is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Antibody (8J512) can be used in WB,ICC/IF,IHC,IP,FCM.

IgG

8J512

Human,Mouse,Rat

1. Western blot analysis of Calreticulin on different lysates using anti-Calreticulin antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y, Lane 2: HL-60.
2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
6. ICC staining Calreticulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Calreticulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Calreticulin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining Calreticulin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. Flow cytometric analysis of Hela cells with Calreticulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein