Anti-Calponin 1+2+3 Polyclonal Antibody is a Rabbit antibody targeting Calponin 1+2+3. Anti-Calponin 1+2+3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse)

1. Positive control: H9C2 (2% Paraformaldehyde-fixed)
Isotype Control Antibody: Rabbit IgG;
Dilution: 1 μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1: 200 in 1 X PBS containing 0.5% BSA
Primary Antibody: rabbit Anti-Calponin 1+2+3 (TMAB-03581);
Dilution: 1 μg in 100 μl 1X PBS containing 0.5% BSA
2. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Calponin 1+2+3) Polyclonal Antibody, Unconjugated (TMAB-03581) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Calponin 1/2/3

Human

CNN2

Calponin-2

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.