Anti-Calpastatin Antibody (9B150) is a Rabbit antibody targeting Calpastatin. Anti-Calpastatin Antibody (9B150) can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

9B150

Human

1. Tissue: Human prostatic hyperplasia
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03580
2. Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03580
3. Cell line: A549
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-03580
4. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for TMAB-03580

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1:50-100

Monoclonal

CAST

Calpastatin

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.