Anti-Calnexin Polyclonal Antibody is a Rabbit antibody targeting Calnexin. Anti-Calnexin Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Cow,Horse,Rabbit)

1. Blank control: H9C2 Cells (blue). Primary Antibody: Rabbit Anti-CANX/FITC Conjugated antibody (TMAB-00270/FITC), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange),used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (TMAB-00270/FITC, 1 μg/1x10^6 cells) for 30 min on ice.
2. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Human K562 cell lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human HepG2 cell lysates
Lane 8: Human U-2 OS cell lysates
Lane 9: Human MCF-7 cell lysates
Lane 10: Human A431 cell lysates
Primary: Anti-Calnexin (TMAB-00270) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 78 kDa

WB,FCM

WB: 1:500-2000; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human CANX

Human

Visual system,ER