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Anti-Calmodulin 1 Antibody (2J470)

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Catalog No. TMAB-03575

Anti-Calmodulin 1 Antibody (2J470) is a Rabbit antibody targeting Calmodulin 1. Anti-Calmodulin 1 Antibody (2J470) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

Anti-Calmodulin 1 Antibody (2J470)

Anti-Calmodulin 1 Antibody (2J470)

Copy Product Info
😃Good
Catalog No. TMAB-03575
Anti-Calmodulin 1 Antibody (2J470) is a Rabbit antibody targeting Calmodulin 1. Anti-Calmodulin 1 Antibody (2J470) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Calmodulin 1 Antibody (2J470) is a Rabbit antibody targeting Calmodulin 1. Anti-Calmodulin 1 Antibody (2J470) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.
Ig Type
IgG
Clone
2J470
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calmodulin 1 Monoclonal Antibody, Unconjugated (TMAB-03575) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. 25 μg total protein per lane of various lysates (see on figure) probed with Calmodulin 1 monoclonal antibody, unconjugated (TMAB-03575) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundCalmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Calcium-binding is required for the activation of calmodulin.
Antigen Details
Immunogen
A synthesized peptide: human Calmodulin 1
Antigen Species
Human
Gene Name
CALM1
Gene ID
Protein Name
Calmodulin-1
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 17 kDa. Actual: 17 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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