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Anti-Caldesmon Antibody (6J397) (Synonyms: NAG22, MGC21352, LCAD, L CAD, HCAD, H CAD, CDM, Caldesmon1, C...)

Catalog No. TMAC-00523 Copy Product Info
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Anti-Caldesmon Antibody (6J397) is a Rabbit antibody targeting Caldesmon. Anti-Caldesmon Antibody (6J397) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-Caldesmon Antibody (6J397)

Copy Product Info
🥰Excellent
Catalog No. TMAC-00523
Synonyms NAG22, MGC21352, LCAD, L CAD, HCAD, H CAD, CDM, Caldesmon1, Caldesmon 1 Isoform 5, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 1, Caldesmon 1, Caldesmon, CALD1_HUMAN, CALD1, CALD 1, CAD

Anti-Caldesmon Antibody (6J397) is a Rabbit antibody targeting Caldesmon. Anti-Caldesmon Antibody (6J397) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-Caldesmon Antibody
(6J397)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4977-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Caldesmon Antibody (6J397) is a Rabbit antibody targeting Caldesmon. Anti-Caldesmon Antibody (6J397) can be used in FCM,ICC/IF,IHC,IP,WB.
SynonymsNAG22, MGC21352, LCAD, L CAD, HCAD, H CAD, CDM, Caldesmon1, Caldesmon 1 Isoform 5, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 1, Caldesmon 1, Caldesmon, CALD1_HUMAN, CALD1, CALD 1, CAD
Ig Type
IgG
Clone
6J397
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Caldesmon on NIH/3T3 cell lysates using anti-Caldesmon antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.
5. ICC staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundCaldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 70 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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