Anti-Caldesmon Antibody (2B731) is a Rabbit antibody targeting Caldesmon. Anti-Caldesmon Antibody (2B731) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2B731

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Lysate: C2C12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 93 kDa
Observed MW: 75 kDa
2. Tissue: Mouse colon
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-03572
3. Tissue: Human colon
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-03572
4. Tissue: rat colon
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-03572

WB: 1:500-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

KLH conjugated synthetic peptide: human Caldesmon

Human

CALD1

Caldesmon

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration.