Anti-Cadherin 16 Antibody (7W191) is a Rabbit antibody targeting Cadherin 16. Anti-Cadherin 16 Antibody (7W191) can be used in IF, IHC-Fr, IHC-P, IP, WB.

IgG

7W191

Human,Mouse

1. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cadherin 16 Monoclonal Antibody, Unconjugated (TMAB-03547) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cadherin 16 Monoclonal Antibody, Unconjugated (TMAB-03547) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. 25 ug total protein per lane of various lysates (see on figure) probed with Cadherin 16 monoclonal antibody, unconjugated (TMAB-03547) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human Cadherin 16

Human

CDH16

Cadherin-16

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.