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Anti-CAD Antibody (5U309)

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Catalog No. TMAB-03544

Anti-CAD Antibody (5U309) is a Rabbit antibody targeting CAD. Anti-CAD Antibody (5U309) can be used in WB,ICC/IF,IP,FCM.

Anti-CAD Antibody (5U309)

Anti-CAD Antibody (5U309)

Copy Product Info
😃Good
Catalog No. TMAB-03544
Anti-CAD Antibody (5U309) is a Rabbit antibody targeting CAD. Anti-CAD Antibody (5U309) can be used in WB,ICC/IF,IP,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CAD Antibody (5U309) is a Rabbit antibody targeting CAD. Anti-CAD Antibody (5U309) can be used in WB,ICC/IF,IP,FCM.
Ig Type
IgG
Clone
5U309
Reactivity
Human
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with CAD monoclonal antibody, unconjugated (TMAB-03544) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CAD antibody (TMAB-03544, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis.
Antigen Details
Immunogen
A synthesized peptide: human CAD
Antigen Species
Human
Gene Name
CAD
Gene ID
Protein Name
Multifunctional protein CAD
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 243 kDa. Actual: 260 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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