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Anti-CA9 Antibody (8W181)

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Catalog No. TMAB-03533

Anti-CA9 Antibody (8W181) is a Rabbit antibody targeting CA9. Anti-CA9 Antibody (8W181) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CA9 Antibody (8W181)

Anti-CA9 Antibody (8W181)

Copy Product Info
😃Good
Catalog No. TMAB-03533
Anti-CA9 Antibody (8W181) is a Rabbit antibody targeting CA9. Anti-CA9 Antibody (8W181) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CA9 Antibody (8W181) is a Rabbit antibody targeting CA9. Anti-CA9 Antibody (8W181) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
8W181
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated (TMAB-03533) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated (TMAB-03533) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with CA9 monoclonal antibody, unconjugated (TMAB-03533) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Tissue SpecificityExpressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundCatalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Antigen Details
Immunogen
A synthesized peptide: human Carbonic anhydrase 9
Antigen Species
Human
Gene Name
CA9
Gene ID
Protein Name
Carbonic anhydrase 9
Uniprot ID
Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Chemical Properties
Molecular WeightTheoretical: 50 kDa. Actual: 50 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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