Anti-C6orf222 Polyclonal Antibody is a Rabbit antibody targeting C6orf222. Anti-C6orf222 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-C6orf222 Polyclonal Antibody, Unconjugated (TMAB-03475) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: Human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-C6orf222 Polyclonal Antibody, Unconjugated (TMAB-03475) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
U937 Cell (Human) Lysate at 30 μg
LOVO Cell (Human) Lysate at 30 μg
A549 Cell (Human) Lysate at 30 μg
Primary: Anti-C6orf222 (TMAB-03475) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human C6orf222

Human

BNIP5

Protein BNIP5