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Anti-C4orf21 Polyclonal Antibody is a Rabbit antibody targeting C4orf21. Anti-C4orf21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
| Description | Anti-C4orf21 Polyclonal Antibody is a Rabbit antibody targeting C4orf21. Anti-C4orf21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Cow,Horse) |
| Verified Activity | 1. Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-C4orf21 Polyclonal Antibody, Unconjugated (TMAB-03443) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: Testis (Mouse) Lysate at 40 μg Molt-4 (Human) Cell Lysate at 30 μg HL60 (Human) Cell Lysate at 30 μg Primary: Anti-C4orf21 (TMAB-03443) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 119 kD Observed band size: 119 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass membrane protein (Potential). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | C4orf12 (chromosome 4 open reading frame 12) is a 926 amino acid single-pass membrane protein that exists as two isoforms. The gene encoding C4orf21 maps to human chromosome 4. Human chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease, is on chromosome 4. FGFR-3 is also encoded by a gene that is located on chromosome 4, and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Immunogen | KLH conjugated synthetic peptide: human C4orf21 |
| Antigen Species | Human |
| Gene Name | ZGRF1 |
| Gene ID | |
| Protein Name | Protein ZGRF1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 105 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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