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Anti-C1QBP Antibody (9T946) is a Rabbit antibody targeting C1QBP. Anti-C1QBP Antibody (9T946) can be used in WB,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $268 | 7-10 days |
Description | Anti-C1QBP Antibody (9T946) is a Rabbit antibody targeting C1QBP. Anti-C1QBP Antibody (9T946) can be used in WB,IP. |
Alias | P32, HABP1, gC1qBP, D11Wsu182e, complement component 1, q subcomponent binding protein, AA986492, AA407365 |
Ig Type | Monoclonal Rabbit IgG |
Clone | 9T946 |
Reactivity | Mouse |
Verified Activity | 1. Mouse C1QBP was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -2 µL anti-Mouse C1QBP rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse C1QBP rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 35 kDa. -Observed band size: 35 kDa. 2. Anti-C1QBP rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: Raji Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:31 kDa. -Observed band size:35 kDa |
Application | WB,IP |
Recommended Dose | WB: 1:500-1:2000; IP: 0.5-2 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse C1QBP / HABP1 (rM C1QBP / HABP1; TMPY-01998; NP_031599.2; Leu72-Gln279). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Hyaluronan binding protein 1 (HABP1), also known as p32 or gC1qR, is a ubiquitously expressed multifunctional phospho-protein implicated in cell signalling. Hyaluronan-binding protein 1 (HABP1) /p32/gC1qR was characterized as a highly acidic and oligomeric protein, which binds to different ligands like hyaluronan, C1q, and mannosylated albumin. The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro. HABP1 overexpressing cells showed extensive vacuolation and reduced growth rate, which was corrected by frequent medium replenishment. Further investigation revealed that HABP1 overexpressing cells undergo apoptosis, and they failed to enter into the S-phase. The sperm surface HABP1 level can be correlated with the degree of sperm motility.Hyaluronan binding protein 1 (HABP1) was reported to be present on human sperm surface and its involvement in fertilization has already been elucidated: decreased HABP1 level may be associated with low motility of sperms, which in turn might cause infertility in the patient. HABP1 also is an endogenous substrate for MAP kinase and upon mitogenic stimulation it is translocated to the nucleus in a MAP kinase-dependent manner. |
Conjucates | Unconjugated |
Immunogen | Recombinant Mouse C1QBP / HABP1 Protein (TMPY-01998) |
Antigen Species | Mouse |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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