Anti-C1QA Polyclonal Antibody is a Rabbit antibody targeting C1QA. Anti-C1QA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat)

1. Sample:
K562 (Human) Cell Lysate at 30 μg
Primary: Anti-C1Q (TMAB-00258) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-C1Q Polyclonal Antibody, Unconjugated (TMAB-00258) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C1Q) Polyclonal Antibody, Unconjugated (TMAB-00258) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:50-200; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human C1QA

Human

Classical Pathway