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Anti-C17orf74 Polyclonal Antibody
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Catalog No. TMAB-03311
Anti-C17orf74 Polyclonal Antibody is a Rabbit antibody targeting C17orf74. Anti-C17orf74 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-C17orf74 Polyclonal Antibody
Anti-C17orf74 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-03311
Anti-C17orf74 Polyclonal Antibody is a Rabbit antibody targeting C17orf74. Anti-C17orf74 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-C17orf74 Polyclonal Antibody is a Rabbit antibody targeting C17orf74. Anti-C17orf74 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-C17orf74 Polyclonal Antibody, Unconjugated (TMAB-03311) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-C17orf74 Polyclonal Antibody, Unconjugated (TMAB-03311) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Sample: A549 Cell (Human) Lysate at 40 μg Primary: Anti-C17orf74 (TMAB-03311) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 63 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single pass membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | C17orf74 is a 501 amino acid protein that is encoded by a gene mapping to human chromosome 17. Chromosome 17 makes up over 2.5% of the human genome with about 81 million bases encoding over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. Chromosome 17 is also linked to neurofibromatosis, a condition characterized by neural and epidermal lesions, and dysregulated Schwann cell growth. Alexander disease, Birt-Hogg-Dube syndrome and Canavan disease are also associated with chromosome 17. |
| Immunogen | KLH conjugated synthetic peptide: human C17orf74 |
| Antigen Species | Human |
| Gene Name | SPEM2 |
| Gene ID | |
| Protein Name | Uncharacterized protein SPEM2 |
| Uniprot ID |
| Molecular Weight | Theoretical: 57 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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