Anti-C-Myc Antibody (3J570) is a Rabbit antibody targeting C-Myc. Anti-C-Myc Antibody (3J570) can be used in WB,FCM,ICC/IF.

IgG1

3J570

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with c-Myc monoclonal antibody, unconjugated (TMAB-04485) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-c-Myc antibody (TMAB-04485): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (c-Myc) monoclonal Antibody, unconjugated (TMAB-04485) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:2000-10000; FCM: 1μg/Test; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human c Myc

MYC

Myc proto-oncogene protein

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.