Anti-C-jun Antibody (2L241) is a Mouse antibody targeting C-jun. Anti-C-jun Antibody (2L241) can be used in WB,ICC/IF.

IgG

2L241

Human

1. Sample:
Lane 1: Human U87MG cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human A431 cell lysates
Lane 4: Recombinant human C-jun protein (50 ng)
Primary: Anti-C-jun (TMAB-04372) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 40,70 kDa
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (C-jun) monoclonal Antibody, Unconjugated (TMAB-04372) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; ICC/IF: 1:100-500

Monoclonal

Recombinant Protein: human C-jun protein

Human

JUN

Transcription factor AP-1

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.