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Anti-C/EBP Beta Antibody (7Q839) is a Rabbit antibody targeting C/EBP Beta. Anti-C/EBP Beta Antibody (7Q839) can be used in WB,ICC/IF,IP,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-C/EBP Beta Antibody (7Q839) is a Rabbit antibody targeting C/EBP Beta. Anti-C/EBP Beta Antibody (7Q839) can be used in WB,ICC/IF,IP,FCM. |
Synonyms | Transcription factor 5, TCF-5, TCF5, Silencer factor B, SFB, SF B, Nuclear factor NF IL6, NFIL6, NF IL6, Liver enriched transcriptional activator, Liver activator protein, LAP, Interleukin 6 dependent binding protein, IL6DBP, IL 6DBP, CRP2, CLEBP beta, CEBPB, CEBP β, CCAAT/enhancer-binding protein beta, CCAAT Enhancer Binding Protein beta, C/EBP related protein 2, C/EBP beta, C EBP beta, AGP/EBP |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: PC-12. 2. ICC staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The C/EBP β form requires phosphorylation for its DNA binding ability, and increase binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 36 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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