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Anti-BubR1 Antibody (8W600)
😃Good
Catalog No. TMAB-03257
Anti-BubR1 Antibody (8W600) is a Rabbit antibody targeting BubR1. Anti-BubR1 Antibody (8W600) can be used in WB,IHC-P,IHC-Fr,IP,FCM,IF.
Anti-BubR1 Antibody (8W600)
Anti-BubR1 Antibody (8W600)
😃Good
Catalog No. TMAB-03257
Anti-BubR1 Antibody (8W600) is a Rabbit antibody targeting BubR1. Anti-BubR1 Antibody (8W600) can be used in WB,IHC-P,IHC-Fr,IP,FCM,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-BubR1 Antibody (8W600) is a Rabbit antibody targeting BubR1. Anti-BubR1 Antibody (8W600) can be used in WB,IHC-P,IHC-Fr,IP,FCM,IF. |
| Ig Type | IgG |
| Clone | 8W600 |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; FCM: 1:50-100; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, centrosome. |
| Tissue Specificity | Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. |
| Immunogen | A synthesized peptide: human BUB1B |
| Antigen Species | Human |
| Gene Name | BUB1B |
| Gene ID | |
| Protein Name | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta |
| Uniprot ID | |
| Function | Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. |
| Molecular Weight | Theoretical: 120 kDa. Actual: 120 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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