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Anti-BTNL2 Polyclonal Antibody
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Catalog No. TMAB-03249
Anti-BTNL2 Polyclonal Antibody is a Rabbit antibody targeting BTNL2. Anti-BTNL2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-BTNL2 Polyclonal Antibody
Anti-BTNL2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-03249
Anti-BTNL2 Polyclonal Antibody is a Rabbit antibody targeting BTNL2. Anti-BTNL2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-BTNL2 Polyclonal Antibody is a Rabbit antibody targeting BTNL2. Anti-BTNL2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Horse,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (BTNL2) Polyclonal Antibody, Unconjugated (TMAB-03249) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-BTNL2 (TMAB-03249) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 52 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type II membrane.protein. Note=Isoform 2 is present in the nuclear, vesicle and.plasma membranes, isoform 3 is found in cytoplasmic vesicle.structures and is not membrane bound. |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, pancreas, ovary, leukocyte, small intestine, testis and thymus.. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Butyrophilin is a glycoprotein that is specifically expressed on the apical surface of mammary epithelial cells during lactation and becomes incorporated as an integral protein into the membrane of the milk fat globule during the budding and secretion of fat droplets into milk. BTNL2 (butyrophilin-like protein 2), also known as BTL-II, is a 455 amino acid single-pass type II membrane protein that acts as a negative regulator of T cell proliferation. Expressed in thymus, brain, kidney, heart, liver, small intestine, testis, ovary, pancreas and leukocytes, BTNL2 exists as at least six alternatively spliced isoforms, which are encoded by a gene located on human chromosome 6. Expression of BTNL2 isoform 3 is associated with susceptibility to sarcoidosis type 2 (SS2), an immune disorder that causes chronic inflammatory granulomatous lesions. |
| Immunogen | KLH conjugated synthetic peptide: human BTNL2 |
| Antigen Species | Human |
| Gene Name | BTNL2 |
| Gene ID | |
| Protein Name | Butyrophilin-like protein 2 |
| Uniprot ID | |
| Function | Negative regulator of T-cell proliferation. |
| Molecular Weight | Theoretical: 50 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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