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Anti-BTN2A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00255
Alias Butyrophilin subfamily 2 member A1, BTN2A1, BTF1, BT2.1

Anti-BTN2A1 Polyclonal Antibody is a Rabbit antibody targeting BTN2A1. Anti-BTN2A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-BTN2A1 Polyclonal Antibody

Anti-BTN2A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00255Alias Butyrophilin subfamily 2 member A1, BTN2A1, BTF1, BT2.1
Anti-BTN2A1 Polyclonal Antibody is a Rabbit antibody targeting BTN2A1. Anti-BTN2A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-BTN2A1 Polyclonal Antibody is a Rabbit antibody targeting BTN2A1. Anti-BTN2A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
AliasButyrophilin subfamily 2 member A1, BTN2A1, BTF1, BT2.1
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-BTN2A1 antibody (TMAB-00255)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control: MCF7 cells (blue). Primary Antibody: Rabbit Anti-BTN2A1 antibody (TMAB-00255), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
3. Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BTN2A1) Polyclonal Antibody, Unconjugated (TMAB-00255) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Research BackgroundThis gene encodes a member of the immunoglobulin superfamily. The gene is located in a cluster of butyrophilin-like genes in the juxta-telomeric region of the major histocompatibility complex on chromosome 6. A pseudogene of this gene has been identified in this cluster. The encoded protein is an integral plasma membrane protein involved in lipid, fatty-acid, and sterol metabolism. Alterations in this gene may be associated with several disease states including metabolic syndrome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human BTN2A1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Lipid metabolism,Channels
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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