Anti-BRN3B Polyclonal Antibody is a Rabbit antibody targeting BRN3B. Anti-BRN3B Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Sheep)

1. Blank control: RSC96 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-BRN3B antibody (TMAB-03223), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BRN3B) Polyclonal Antibody, Unconjugated (TMAB-03223) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human BRN3B

Human

POU4F2

POU domain, class 4, transcription factor 2

Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.