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Anti-BrdU Antibody (8W188)
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Catalog No. TMAC-00474
Alias Budr, Bromodeoxyuridine
Anti-BrdU Antibody (8W188) is a Mouse antibody targeting BrdU. Anti-BrdU Antibody (8W188) can be used in FCM,IF,IHC-P.
Anti-BrdU Antibody (8W188)
Anti-BrdU Antibody (8W188)
😃Good
Catalog No. TMAC-00474Alias Budr, Bromodeoxyuridine
Anti-BrdU Antibody (8W188) is a Mouse antibody targeting BrdU. Anti-BrdU Antibody (8W188) can be used in FCM,IF,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $427 | 7-10 days |
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Chemical Properties
| Description | Anti-BrdU Antibody (8W188) is a Mouse antibody targeting BrdU. Anti-BrdU Antibody (8W188) can be used in FCM,IF,IHC-P. |
| Synonyms | Budr, Bromodeoxyuridine |
| Clone | 8W188 |
| Reactivity | Bromodeoxyuridine |
| Verified Activity | 1. Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization. 2. Intracellular FCM analysis of fixed and permeabilized untreated (dotted blue histogram) and BrdU treated (solid orange histogram) K-562 cells. Green line histogram represents the isotype control, normal mouse IgG1. |
| Application | |
| Recommended Dose | IHC-P: 1:50-500; FCM: 1 μg per 1 x 106 cells |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid. |
| Conjucates | Unconjugated |
| Immunogen | Peptide |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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