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Anti-BrdU Antibody (8W188) (Synonyms: Budr, Bromodeoxyuridine)

Catalog No. TMAC-00474 Copy Product Info
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Anti-BrdU Antibody (8W188) is a Mouse antibody targeting BrdU. Anti-BrdU Antibody (8W188) can be used in FCM,IF,IHC-P.

Anti-BrdU Antibody (8W188)

Copy Product Info
🥰Excellent
Catalog No. TMAC-00474
Synonyms Budr, Bromodeoxyuridine

Anti-BrdU Antibody (8W188) is a Mouse antibody targeting BrdU. Anti-BrdU Antibody (8W188) can be used in FCM,IF,IHC-P.

Anti-BrdU Antibody
(8W188)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2967-10 days7-10 days
100 μL$4277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-BrdU Antibody (8W188) is a Mouse antibody targeting BrdU. Anti-BrdU Antibody (8W188) can be used in FCM,IF,IHC-P.
SynonymsBudr, Bromodeoxyuridine
Clone
8W188
Reactivity
Bromodeoxyuridine
Verified Activity
1. Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization.
2. Intracellular FCM analysis of fixed and permeabilized untreated (dotted blue histogram) and BrdU treated (solid orange histogram) K-562 cells. Green line histogram represents the isotype control, normal mouse IgG1.
verifiedActivityverifiedActivity
Application
Recommended Dose
IHC-P: 1:50-500; FCM: 1 μg per 1 x 106 cells
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Peptide
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 12 months, do not freeze.
TransportShipping with blue ice.

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