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Anti-BRD2 Antibody (7Q375)

😃Good
Catalog No. TMAC-00473
Alias RNF3, RING3 PROTEIN, RING3, Really interesting new gene 3 protein, O27.1.1, NAT, KIAA9001, FSRG1, FSH, FLJ31942, Female sterile homeotic related gene 1, mouse, homolog of, Female sterile homeotic related gene 1, DKFZp686N0336, D6S113E, Bromodomain-containing protein 2, Bromodomain containing 2, BRD2_HUMAN, Brd2, BRD 2

Anti-BRD2 Antibody (7Q375) is a Rabbit antibody targeting BRD2. Anti-BRD2 Antibody (7Q375) can be used in WB,ICC,IHC,FCM.

Anti-BRD2 Antibody (7Q375)

Anti-BRD2 Antibody (7Q375)

😃Good
Catalog No. TMAC-00473Alias RNF3, RING3 PROTEIN, RING3, Really interesting new gene 3 protein, O27.1.1, NAT, KIAA9001, FSRG1, FSH, FLJ31942, Female sterile homeotic related gene 1, mouse, homolog of, Female sterile homeotic related gene 1, DKFZp686N0336, D6S113E, Bromodomain-containing protein 2, Bromodomain containing 2, BRD2_HUMAN, Brd2, BRD 2
Anti-BRD2 Antibody (7Q375) is a Rabbit antibody targeting BRD2. Anti-BRD2 Antibody (7Q375) can be used in WB,ICC,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-BRD2 Antibody (7Q375) is a Rabbit antibody targeting BRD2. Anti-BRD2 Antibody (7Q375) can be used in WB,ICC,IHC,FCM.
SynonymsRNF3, RING3 PROTEIN, RING3, Really interesting new gene 3 protein, O27.1.1, NAT, KIAA9001, FSRG1, FSH, FLJ31942, Female sterile homeotic related gene 1, mouse, homolog of, Female sterile homeotic related gene 1, DKFZp686N0336, D6S113E, Bromodomain-containing protein 2, Bromodomain containing 2, BRD2_HUMAN, Brd2, BRD 2
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin.
4. ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining BRD2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining BRD2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomain-containing proteins are ubiquitously expressed.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: human BRD2 aa 1-200
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 88 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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