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Anti-BRD2 Antibody (7Q375) is a Rabbit antibody targeting BRD2. Anti-BRD2 Antibody (7Q375) can be used in FCM,ICC,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
| Description | Anti-BRD2 Antibody (7Q375) is a Rabbit antibody targeting BRD2. Anti-BRD2 Antibody (7Q375) can be used in FCM,ICC,IHC,WB. |
| Synonyms | RNF3, RING3 PROTEIN, RING3, Really interesting new gene 3 protein, O27.1.1, NAT, KIAA9001, FSRG1, FSH, FLJ31942, Female sterile homeotic related gene 1, mouse, homolog of, Female sterile homeotic related gene 1, DKFZp686N0336, D6S113E, Bromodomain-containing protein 2, Bromodomain containing 2, BRD2_HUMAN, Brd2, BRD 2 |
| Ig Type | IgG |
| Clone | 7Q375 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin. 4. ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining BRD2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining BRD2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomain-containing proteins are ubiquitously expressed. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human BRD2 aa 1-200 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 88 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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