Anti-BRD2 Antibody (3E992) is a Rabbit antibody targeting BRD2. Anti-BRD2 Antibody (3E992) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

3E992

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRD2  Monoclonal Antibody, Unconjugated (TMAB-03213) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. 25 ug total protein per lane of various lysates (see on figure) probed with BRD2 monoclonal antibody, unconjugated (TMAB-03213) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-BRD2 antibody (TMAB-03213, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (BRD2) monoclonal Antibody, unconjugated (TMAB-03213) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

A synthesized peptide: human BRD2

Human

BRD2

Bromodomain-containing protein 2

May play a role in spermatogenesis or folliculogenesis.