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Anti-BRCC3 Polyclonal Antibody is a Rabbit antibody targeting BRCC3. Anti-BRCC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-BRCC3 Polyclonal Antibody is a Rabbit antibody targeting BRCC3. Anti-BRCC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Lys-63-specific deubiquitinase BRCC36, CXorf53, C6.1A, BRISC complex subunit BRCC36, BRCC36, BRCC3, BRCA1-A complex subunit BRCC36, BRCA1/BRCA2-containing complex subunit 36, BRCA1/BRCA2-containing complex subunit 3 |
Ig Type | IgG |
Reactivity | Human (predicted:Mouse,Rat,Cow,Rabbit) |
Verified Activity | 1. Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated (TMAB-00249) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-DEPDC1B/BRCC3 (TMAB-00249) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 68 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | intracellular |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | GTPase activator activity. biological process: intracellular signaling cascade |
Immunogen | KLH conjugated synthetic peptide: human DEPDC1B/BRCC3 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Regulators |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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