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Anti-BRAF35 Polyclonal Antibody
Catalog No. TMAB-03202 Copy Product Info
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Anti-BRAF35 Polyclonal Antibody is a Rabbit antibody targeting BRAF35. Anti-BRAF35 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-BRAF35 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-03202
Anti-BRAF35 Polyclonal Antibody is a Rabbit antibody targeting BRAF35. Anti-BRAF35 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-BRAF35 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-BRAF35 Polyclonal Antibody is a Rabbit antibody targeting BRAF35. Anti-BRAF35 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Cow) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BRAF35) Polyclonal Antibody, Unconjugated (TMAB-03202) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2. |
| Tissue Specificity | Ubiquitously expressed in adult tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The breast cancer susceptibility gene (BRCA1) localizes to chromosome 17q. Mutations within this gene account for approximately 45% of families with high incidence of breast cancer and at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2, located on chromosome 13q12-13, also confers a high incidence of breast cancer, but unlike BRCA1, BRCA2 does not confer a substantially elevated risk of ovarian cancer. The BRCA2-Associated Factor 35 (BRAF35) protein forms a complex with BRCA2, which associates with condensed chromatin during histone H3 phosphorylation. BRAF35 expression levels are highest in proliferating tissues and parallel BRCA2 expression patterns. The structure of BRAF35 includes a kinesin-like coiled coil domain and a nonspecific DNA binding HMG domain. The chromatin localization of BRAF35 and antibody microinjection studies indicate a role for the BRAF35/BRCA2 complex in cell cycle regulation. |
| Immunogen | KLH conjugated synthetic peptide: human BRAF35 |
| Antigen Species | Human |
| Gene Name | HMG20B |
| Gene ID | |
| Protein Name | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related |
| Uniprot ID | |
| Function | Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters. |
| Molecular Weight | Theoretical: 36 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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