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Anti-BRAF Antibody (7W36) (Synonyms: v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein k...)

Catalog No. TMAC-00357 Copy Product Info
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Anti-BRAF Antibody (7W36) is a Rabbit antibody targeting BRAF. Anti-BRAF Antibody (7W36) can be used in FCM,IHC,IP,WB.

Anti-BRAF Antibody (7W36)

Copy Product Info
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Catalog No. TMAC-00357
Synonyms v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p94, EC 2.7.11.1, BRAF1

Anti-BRAF Antibody (7W36) is a Rabbit antibody targeting BRAF. Anti-BRAF Antibody (7W36) can be used in FCM,IHC,IP,WB.

Anti-BRAF Antibody
(7W36)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-BRAF Antibody (7W36) is a Rabbit antibody targeting BRAF. Anti-BRAF Antibody (7W36) can be used in FCM,IHC,IP,WB.
Synonymsv-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p94, EC 2.7.11.1, BRAF1
Ig Type
IgG
Clone
7W36
Reactivity
Human,Mouse,Rat
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-B Raf antibody. Counter stained with hematoxylin.
4. Flow cytometric analysis of Hela cells with B Raf antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000; IHC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundSeveral serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 85 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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