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Anti-BRAF Antibody (7W36)
😃Good
Catalog No. TMAC-00357
Alias v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p94, EC 2.7.11.1, BRAF1
Anti-BRAF Antibody (7W36) is a Rabbit antibody targeting BRAF. Anti-BRAF Antibody (7W36) can be used in FCM,IHC,IP,WB.
Anti-BRAF Antibody (7W36)
Anti-BRAF Antibody (7W36)
😃Good
Catalog No. TMAC-00357Alias v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p94, EC 2.7.11.1, BRAF1
Anti-BRAF Antibody (7W36) is a Rabbit antibody targeting BRAF. Anti-BRAF Antibody (7W36) can be used in FCM,IHC,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-BRAF Antibody (7W36) is a Rabbit antibody targeting BRAF. Anti-BRAF Antibody (7W36) can be used in FCM,IHC,IP,WB. |
| Synonyms | v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p94, EC 2.7.11.1, BRAF1 |
| Ig Type | IgG |
| Clone | 7W36 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-B Raf antibody. Counter stained with hematoxylin. 4. Flow cytometric analysis of Hela cells with B Raf antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 85 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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