Anti-BRAF Antibody (1C540) is a Rabbit antibody targeting BRAF. Anti-BRAF Antibody (1C540) can be used in WB,IHC-P,IHC-Fr,IF,IP,FCM,ICC/IF.

IgG

1C540

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRAF Monoclonal Antibody, Unconjugated (TMAB-03201) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRAF Monoclonal Antibody, Unconjugated (TMAB-03201) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRAF Monoclonal Antibody, Unconjugated (TMAB-03201) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRAF Monoclonal Antibody, Unconjugated (TMAB-03201) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRAF Monoclonal Antibody, Unconjugated (TMAB-03201) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRAF Monoclonal Antibody, Unconjugated (TMAB-03201) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. 25 μg total protein per lane of various lysates (see on figure) probed with BRAF monoclonal antibody, unconjugated (TMAB-03201) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
8. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (BRAF) monoclonal Antibody, unconjugated (TMAB-03201) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human B Raf

Human

BRAF

Serine/threonine-protein kinase B-raf

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.