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Anti-Brachyury/TBXT Antibody (6H365)
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Catalog No. TMAY-00125
Alias TFT, T, SAVA
Anti-Brachyury/TBXT Antibody (6H365) is a Mouse antibody targeting Brachyury/TBXT. Anti-Brachyury/TBXT Antibody (6H365) can be used in ICC/IF.
Anti-Brachyury/TBXT Antibody (6H365)
Anti-Brachyury/TBXT Antibody (6H365)
😃Good
Catalog No. TMAY-00125Alias TFT, T, SAVA
Anti-Brachyury/TBXT Antibody (6H365) is a Mouse antibody targeting Brachyury/TBXT. Anti-Brachyury/TBXT Antibody (6H365) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ICC/IF Reactivity: Human |
| Alias | TFT, T, SAVA |
| Ig Type | Mouse IgG2b |
| Clone | 6H365 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of TBXT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human T monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Nucleus. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Brachyury/TBXT. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Brachyury is encoded by the TBXT gene. TBXT plays in the Cnidaria, appears to be in defining the blastopore. Brachyury is an important factor in promoting the epithelial-mesenchymal transition (EMT). Cells that over-express brachyury have down-regulated expression of the adhesion molecule E-cadherin, which allows them to undergo EMT. This process is at least partially mediated by the transcription factors AKT and Snail. Brachyury is overexpressed in a number of tumor types. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: C-terminus of the Human Brachyury/TBXT |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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