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Anti-BNIP1 Antibody (4L976)

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Catalog No. TMAB-03177 Copy Product Info
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Anti-BNIP1 Antibody (4L976) is a Rabbit antibody targeting BNIP1. Anti-BNIP1 Antibody (4L976) can be used in FCM, WB.

Anti-BNIP1 Antibody
(4L976)

Pack SizePriceUSA StockGlobal StockQuantity
25 µL$1517-10 days7-10 days
50 µL$2647-10 days7-10 days
100 µL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-BNIP1 Antibody (4L976) is a Rabbit antibody targeting BNIP1. Anti-BNIP1 Antibody (4L976) can be used in FCM, WB.
Ig Type
IgG
Clone
4L976
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with BNIP1 monoclonal antibody, unconjugated (TMAB-03177) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A431 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-BNIP1 antibody (TMAB-03177, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
FCM=1:50-100; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion. Endoplasmic reticulum membrane; Single-pass type IV membrane protein.
Tissue SpecificityIsoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundSNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death.
Antigen Details
Immunogen
A synthesized peptide: human BNIP1
Antigen Species
Human
Gene Name
BNIP1
Gene ID
Protein Name
Vesicle transport protein SEC20
Uniprot ID
Function
SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy.
Chemical Properties
Molecular WeightTheoretical: 26 kDa. Actual: 26 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-BNIP1 Antibody (4L976) molecular weight