Anti-BNIP1 Antibody (4L976) is a Rabbit antibody targeting BNIP1. Anti-BNIP1 Antibody (4L976) can be used in FCM, WB.

IgG

4L976

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with BNIP1 monoclonal antibody, unconjugated (TMAB-03177) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A431 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-BNIP1 antibody (TMAB-03177, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; WB=1:500-2000

Monoclonal

A synthesized peptide: human BNIP1

Human

BNIP1

Vesicle transport protein SEC20

SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy.