Anti-BMP6 Antibody (3A931) is a Rabbit antibody targeting BMP6. Anti-BMP6 Antibody (3A931) can be used in WB,ICC/IF.

IgG/Kappa

3A931

Human,Mouse,Rat

1. Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-03165
2. Cell line: U-2 OS
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-03165
3. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: A549, SH-SY5Y, N2a, Rat lung
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 42 kDa
Observed MW: 42 kDa
4. SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (BMP6) monoclonal Antibody, Unconjugated (TMAB-03165) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-1000; ICC/IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human BMP6

Human

BMP6

Bone morphogenetic protein 6

Induces cartilage and bone formation.