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Anti-BMP-7 Polyclonal Antibody
😃Good
Catalog No. TMAB-00245
Anti-BMP-7 Polyclonal Antibody is a Rabbit antibody targeting BMP-7. Anti-BMP-7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-BMP-7 Polyclonal Antibody
Anti-BMP-7 Polyclonal Antibody
😃Good
Catalog No. TMAB-00245
Anti-BMP-7 Polyclonal Antibody is a Rabbit antibody targeting BMP-7. Anti-BMP-7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat,Rabbit (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,Rabbit (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: 293T Cell (Human) Lysate at 30 μg Primary: Anti-BMP7 (TMAB-00245) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15.7/47 kDa Observed band size: 49/40 kDa 2. Tissue/cell: rabbit alveolar bone; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-BMP7 Polyclonal Antibody, Unconjugated (TMAB-00245) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Bone (Mouse) Lysate at 40 μg Primary: Anti-BMP7 (TMAB-00245) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15.7/47 kDa Observed band size: 47,50 kDa 4. Sample: Lane 1: Kidney (Mouse) Lysate at 40 μg Lane 2: Placenta (Mouse) Lysate at 40 μg Lane 3: Cerebrum (Mouse) Lysate at 40 μg Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 5: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-BMP7 (TMAB-00245) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human BMP7 |
| Antigen Species | Human |
| Gene ID | 655 |
| Uniprot ID | |
| Biology Area | Cell differentiation,Cell differentiation,Kidney development,Nervous system development,Lipid metabolism,Bone,TGF,Secreted |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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