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Anti-BMP-4 Antibody (8O817)

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Catalog No. TMAY-01024
Alias ZYME, OFC11, MCOPS6, bone morphogenetic protein 4, BMP2B1, BMP2B

Anti-BMP-4 Antibody (8O817) is a Mouse antibody targeting BMP-4. Anti-BMP-4 Antibody (8O817) can be used in FCM,ICC/IF.

Anti-BMP-4 Antibody (8O817)

Anti-BMP-4 Antibody (8O817)

😃Good
Catalog No. TMAY-01024Alias ZYME, OFC11, MCOPS6, bone morphogenetic protein 4, BMP2B1, BMP2B
Anti-BMP-4 Antibody (8O817) is a Mouse antibody targeting BMP-4. Anti-BMP-4 Antibody (8O817) can be used in FCM,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-BMP-4 Antibody (8O817) is a Mouse antibody targeting BMP-4. Anti-BMP-4 Antibody (8O817) can be used in FCM,ICC/IF.
AliasZYME, OFC11, MCOPS6, bone morphogenetic protein 4, BMP2B1, BMP2B
Ig Type
Monoclonal Mouse IgM
Clone
8O817
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human BMP4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BMP4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human BMP4 on HeLa cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human BMP4, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundBMP4 (bone morphogenetic protein-4)-directed differentiation of human embryonic stem cells (ESCd). Autophagy is a conserved catabolic process with complicated roles in tumor development. Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor (TGF-β) family of regulatory proteins, plays a crucial role in human malignancies. BMP4 treatment promoted HCC cells proliferation and induced autophagy. Mechanistic study revealed that the induction of autophagy by BMP4 was mediated through activating the JNK1/Bcl2 pathway. And the JNK1 inhibitor and knockdown of JNK1 could attenuate autophagy induced by BMP4 and eliminated BMP4-promoted HCC cells growth. BMP4 promoted HCC proliferation by autophagy activation through JNK1/Bcl-2 signaling.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human BMP-4 protein
Antigen Species
Human
Biology Area
Early Mesodermal Lineage Markers, Cardiac Stem Cell Markers
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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