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Anti-BMP-2 Antibody (1M274)
😃Good
Catalog No. TMAY-00808
Alias bone morphogenetic protein 2
Anti-BMP-2 Antibody (1M274) is a Mouse antibody targeting BMP-2. Anti-BMP-2 Antibody (1M274) can be used in FCM,ICC/IF.
Anti-BMP-2 Antibody (1M274)
Anti-BMP-2 Antibody (1M274)
😃Good
Catalog No. TMAY-00808Alias bone morphogenetic protein 2
Anti-BMP-2 Antibody (1M274) is a Mouse antibody targeting BMP-2. Anti-BMP-2 Antibody (1M274) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: FCM, ICC/IF Reactivity: Human, Mouse, Rat, Rhesus, Canine |
| Alias | bone morphogenetic protein 2 |
| Ig Type | Mouse IgG1 |
| Clone | 1M274 |
| Reactivity | Human, Mouse, Rat, Rhesus, Canine |
| Verified Activity | 1. Immunofluorescence staining of Human BMP2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BMP2 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human BMP2 expression on Hela cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human BMP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human / Mouse / Rat / Rhesus / Canine BMP-2 (rh BMP-2; TMPY-01136; NP_001191.1; Gln283-Arg396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human / Mouse / Rat / Rhesus / Canine BMP-2 protein (TMPY-01136) |
| Antigen Species | Human, Mouse, Rat, Rhesus, Canine |
| Biology Area | Early Mesodermal Lineage Markers |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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