Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BMP1 Polyclonal Antibody, Unconjugated (TMAB-00240) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-BMP1 (TMAB-00240) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
3. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human A549 cell lysates
Primary: Anti-BMP1 (TMAB-00240) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human BMP1

Human

649

Metabolism of lipids and lipoproteins,Cell differentiation,BMPs,Lipid metabolism,MMP,Collagen,Bone