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Anti-BMAL1 Antibody (5D959)
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Catalog No. TMAC-00453
Alias TIC, PASD3, PAS domain-containing protein 3, MOP3, Member of PAS protein 3, Class E basic helix-loop-helix protein 5(bHLHe5), Brain and muscle ARNT-like 1, BMAL1c, BMAL1, bHLH-PAS protein JAP3, BHLHE5, Basic-helix-loop-helix-PAS protein MOP3, Basic helix-loop-helix ARNT-like protein 1, Aryl hydrocarbon receptor nuclear translocator-like protein 1, ARNTL1, ARNTL
Anti-BMAL1 Antibody (5D959) is a Rabbit antibody targeting BMAL1. Anti-BMAL1 Antibody (5D959) can be used in WB,ICC/IF.
Anti-BMAL1 Antibody (5D959)
Anti-BMAL1 Antibody (5D959)
😃Good
Catalog No. TMAC-00453Alias TIC, PASD3, PAS domain-containing protein 3, MOP3, Member of PAS protein 3, Class E basic helix-loop-helix protein 5(bHLHe5), Brain and muscle ARNT-like 1, BMAL1c, BMAL1, bHLH-PAS protein JAP3, BHLHE5, Basic-helix-loop-helix-PAS protein MOP3, Basic helix-loop-helix ARNT-like protein 1, Aryl hydrocarbon receptor nuclear translocator-like protein 1, ARNTL1, ARNTL
Anti-BMAL1 Antibody (5D959) is a Rabbit antibody targeting BMAL1. Anti-BMAL1 Antibody (5D959) can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-BMAL1 Antibody (5D959) is a Rabbit antibody targeting BMAL1. Anti-BMAL1 Antibody (5D959) can be used in WB,ICC/IF. |
| Synonyms | TIC, PASD3, PAS domain-containing protein 3, MOP3, Member of PAS protein 3, Class E basic helix-loop-helix protein 5(bHLHe5), Brain and muscle ARNT-like 1, BMAL1c, BMAL1, bHLH-PAS protein JAP3, BHLHE5, Basic-helix-loop-helix-PAS protein MOP3, Basic helix-loop-helix ARNT-like protein 1, Aryl hydrocarbon receptor nuclear translocator-like protein 1, ARNTL1, ARNTL |
| Ig Type | IgG |
| Clone | 5D959 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3, Lane 2: Rat brain, Lane 3: Mouse spleen, Lane 4: Hela. 2. ICC staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Transcriptional activator which forms a core component of the circadian clock. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 68 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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