Anti-BLBP Antibody (6C759) is a Mouse antibody targeting BLBP. Anti-BLBP Antibody (6C759) can be used in WB,IHC-P,IF,IHC-Fr.

IgG1

6C759

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Rat cerebellum;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (TMAB-03153) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat brain;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (TMAB-03153) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. (Negative control) paraformaldehyde-fixed, paraffin embedded Rat kidney;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (TMAB-03153) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse brain;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (TMAB-03153) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human glioma;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (TMAB-03153) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Brain;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (TMAB-03153) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
7. (Negative control) paraformaldehyde-fixed, paraffin embedded Human kidney;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (TMAB-03153) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
8. 25 μg total protein per lane of various lysates (see on figure) probed with BLBP monoclonal antibody, unconjugated (TMAB-03153) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:500-2000; IF: 1:500-2000; IHC-Fr: 1:500-2000

Monoclonal

Recombinant Protein: Mouse BLBP protein

Mouse

FABP7

Fatty acid-binding protein, brain

FABP are thought to play a role in the intracellular transport of long chain fatty acids and their acyl-CoA esters. Binds oleic and palmitic acids but not palmitoyl CoA.