Anti-Bim Polyclonal Antibody 2 is a Rabbit antibody targeting Bim. Anti-Bim Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Sheep)

1. Sample:
Raji (Human) Cell Lysate at 30 μg
Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-Bim (TMAB-03146) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 25 kD
2. Sample:
LOVO (Human) Cell Lysate at 30 μg
Primary: Anti-Bim (TMAB-03146) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 25 kD
3. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-Bim antibody (TMAB-03146)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: the middle of human Bim

Human

BCL2L11

Bcl-2-like protein 11

Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.