Anti-Biglycan Polyclonal Antibody is a Rabbit antibody targeting Biglycan. Anti-Biglycan Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (TMAB-03143) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Colon (Rat) Lysate at 40 μg
Embryo (Mouse) Lysate at 40 μg
Primary: Anti-Biglycan (TMAB-03143) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 37 kD
Observed band size: 52 kD
3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated (TMAB-03143) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Sample: Bone (mouse) Lysate at 40 μg
Primary: Anti-Biglycan (TMAB-03143) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 52 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Biglycan

Human

BGN

Biglycan

May be involved in collagen fiber assembly (By similarity).