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Anti-Beta-Catenin Antibody (5O621)
😃Good
Catalog No. TMAB-00229
Alias PRO2286, OK/SW-cl.35, NEDSDV, MRD19, EVR7, CTNNB, beta-catenin, armadillo
Anti-Beta-Catenin Antibody (5O621) is a Mouse antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (5O621) can be used in WB.
Anti-Beta-Catenin Antibody (5O621)
Anti-Beta-Catenin Antibody (5O621)
😃Good
Catalog No. TMAB-00229Alias PRO2286, OK/SW-cl.35, NEDSDV, MRD19, EVR7, CTNNB, beta-catenin, armadillo
Anti-Beta-Catenin Antibody (5O621) is a Mouse antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (5O621) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Beta-Catenin Antibody (5O621) is a Mouse antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (5O621) can be used in WB. |
| Alias | PRO2286, OK/SW-cl.35, NEDSDV, MRD19, EVR7, CTNNB, beta-catenin, armadillo |
| Ig Type | IgG |
| Clone | 5O621 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: U87Mg (Human) Cell Lysate at 30 μg U2os (Human) Cell Lysate at 30 μg Primary: Anti-Beta catenin (TMAB-00229) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 92 kDa 2. Sample: Hela (Human) Cell Lysate at 30 μg NIH/3T3 (Mouse) Cell Lysate at 30 μg Medulld (Mouse) Lysate at 40 μg Testis (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Primary: Anti-Beta catenin (TMAB-00229) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 95 kDa |
| Application | |
| Recommended Dose | WB: 1:1000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]. |
| Immunogen | Recombinant Protein: human Beta catenin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cytoplasmic,Catenins,Cadherins,beta catenin,Neurogenesis,ChIP antibodies,Leukocyte recruitment,Cadherins |
| Molecular Weight | Theoretical: 92 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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