Anti-Benzoyllysine Antibody (6T433) is a Mouse antibody targeting Benzoyllysine. Anti-Benzoyllysine Antibody (6T433) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

6T433

Species independent

1. Tissue: mouse liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03085
2. Tissue: rat brain
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03085
3. Tissue: human testis
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03085
4. Tissue: human placenta
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03085
5. Sealing solution and concentration: 5% NFDM/TBST
First antibody dilution: 1:1000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti Mouse IgG H&L (HRP)
Cracking liquid: HeLa, N2a, HepG2
Protein loading amount: 20 μg
Exposure time: 60 seconds
Theoretical molecular weight: Multiple kDa
Actual molecular weight: Multiple kDa

WB: 1:500-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

benzoylated BSA