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Anti-BCLG Antibody (4H668)
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Catalog No. TMAB-03068
Anti-BCLG Antibody (4H668) is a Mouse antibody targeting BCLG. Anti-BCLG Antibody (4H668) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-BCLG Antibody (4H668)
Anti-BCLG Antibody (4H668)
Copy Product Info😃Good
Catalog No. TMAB-03068
Anti-BCLG Antibody (4H668) is a Mouse antibody targeting BCLG. Anti-BCLG Antibody (4H668) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-BCLG Antibody (4H668) is a Mouse antibody targeting BCLG. Anti-BCLG Antibody (4H668) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG/Kappa |
| Clone | 4H668 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: A549, HepG2, Rat brain, Mouse pancreas Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 37 kDa Observed MW: 52 kDa 2. Tissue: Human colon cancer Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-03068 3. Tissue: Mouse stomach Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-03068 4. Tissue: Rat lung Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-03068 5. Cell line: A549 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for TMAB-03068 |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Isoform 1: Cytoplasm, cytosol. Note=Diffusely distributed throughout the cytosol. Isoform 2: Endomembrane system. Note=Predominantly localized to cytosolic organelles. |
| Tissue Specificity | Isoform 1 is widely expressed. Isoform 2 is testis-specific. |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Bcl-G is a novel pro-apoptotic member of the Bcl-2 family. The human Bcl-G gene encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L). |
| Gene Name | BCL2L14 |
| Gene ID | |
| Protein Name | Apoptosis facilitator Bcl-2-like protein 14 |
| Function | Plays a role in apoptosis. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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