Anti-BCLAF1 Antibody (2I249) is a Rabbit antibody targeting BCLAF1. Anti-BCLAF1 Antibody (2I249) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

2I249

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with BCLAF1 monoclonal antibody, unconjugated (TMAB-03067) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (BCLAF1) monoclonal Antibody, unconjugated (TMAB-03067) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BCLAF1 Monoclonal Antibody, Unconjugated (TMAB-03067) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BCLAF1 Monoclonal Antibody, Unconjugated (TMAB-03067) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BCLAF1 Monoclonal Antibody, Unconjugated (TMAB-03067) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BCLAF1 Monoclonal Antibody, Unconjugated (TMAB-03067) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BCLAF1 Monoclonal Antibody, Unconjugated (TMAB-03067) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human BTF

Human

BCLAF1

Bcl-2-associated transcription factor 1

Death-promoting transcriptional repressor.