Anti-BCL6 Antibody (7P602) is a Rabbit antibody targeting BCL6. Anti-BCL6 Antibody (7P602) can be used in FCM,ICC/IF.

IgG

7P602

Human

1. The Daudi (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-BCL6 antibody (TMAB-03065): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed Daudi (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (BCL6) monoclonal Antibody, unconjugated (TMAB-03065) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM: 1:50-100; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human BCL 6

BCL6

B-cell lymphoma 6 protein

Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis.