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Anti-BCL2 Antibody (6L780)
😃Good
Catalog No. TMAY-00433
Alias PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (6L780) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (6L780) can be used in WB,IP.
Anti-BCL2 Antibody (6L780)
Anti-BCL2 Antibody (6L780)
😃Good
Catalog No. TMAY-00433Alias PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (6L780) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (6L780) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, IP Reactivity: Human |
| Alias | PPP1R50, Bcl-2, B-cell CLL/lymphoma 2 |
| Ig Type | Mouse IgG1 |
| Clone | 6L780 |
| Reactivity | Human |
| Verified Activity | 1. Anti-BCL2 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: U937 Whole Cell Lysate. -Lane D: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:27 kDa. 2. BCL2 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate. -2 µL anti-BCL2 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-BCL2 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 20 kDa. -Observed band size: 20 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BCL2 / Bcl-2 (rh BCL2 / Bcl-2; TMPY-02700; P10415-1; Met1-Asp211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human BCL2 / Bcl-2 protein (TMPY-02700) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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