Anti-BCL2 Antibody (5A806) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (5A806) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG1

5A806

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (TMAB-00195) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (TMAB-00195) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (TMAB-00195) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Sample:
LOVO (Human) Cell Lysate at 30 μg
Primary: Anti-Bcl-2 (TMAB-00195) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
5. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
6. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (TMAB-00195) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (TMAB-00195) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (TMAB-00195) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
9. Sample:
LOVO Cell (Human) Lysate at 40 μg
MCF-7 Cell (Human) Lysate at 40 μg
Primary: Anti-Bcl-2 (TMAB-00195) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa

ICC/IF=1:100-500; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

KLH conjugated synthetic peptide: human Bcl-2

Human

BCL2

Apoptosis regulator Bcl-2

Cellular metabolic process,Response to hypoxia,Bcl 2 family,Metabolism,Bcl 2 family,Cell survival & death,Oncoproteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis,Hypoxia,Mitochondrial markers,Cancer,Mitochondrial

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).